Anti-oxidant effect proved by Nitric oxide assay, DPPH assay
Psoronorm cell repair oil decreases cell senescence which leads to DNA damage in response to elevated reactive oxygen species (ROS). This benefit is desired for Psoriatic skin.
DPPH ASSAY
| Psoronorm cell repair oil | IC 50 value at 50 mg/ml |
| Absorbic acid (positive control) | IC 50 value at 1.52 mg/ml |
Inhibits DPPH with IC 50 value of 50 mg/ml
INHIBITION OF NITRIC OXIDE (NO) PRODUCTION
| Test Details | % of Nitrate Production |
| Only LPS (Positive Control) | 73 |
| LPS + 50 mg of Psoronorm cell repair oil | 39 |
| Untreated cells | 22 |
Inhibits the production of NO with IC 50 value of 39 mg/ml
Elastase inhibition assay,collagenase inhibition assay,anti glycation assay, Prove that Psoronorm cell repair oil
- Acts against Elastase and Collagenase enzymes which worsen dry skin conditions.
- Possess Anti - glycation effect to retard free radicals and inflammatory responses of the skin.
Ellastase Assay
| Conc of Product mg/ml | % inhibition of Elastase |
| 50 | 18 |
| 40 | 11 |
Anti-glycation Assay
| Psoronorm cell repair oil | 75 mg/ml |
| Arbutin (positive control) | 2.0 mg/ml |
Possess Anti - glycation effect to retard free radicals and inflammatory responses of the skin.
Collagenase inhibition Assay
| Products | Concentration | % inhibition of collagenase |
| Psoronorm cell repair oil | 50 mg/ml | 11 % |
| Epi gallo catechin gallate (positive control) | 250 um (0.114 mg/ml) | 40 % |
Showed collagenase inhibition at 50 mg/ml
Prolonged use of cell repair oil is proven to be safe as established by fibrobalst toxicity assay and zein protein hydrolysis assay.
Fibroblast toxicity test- MTT assay
Cell repair oil inhibited fibroblast by 9% at 50 mg/ml.
Zein protein hydrolysis assay
Cell repair oil hydrolyzed zein protein by 9% as against SLS at 90%
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